Interaction Details


Details of experiments between P36507 and P53667
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Protein B

Protein: LIMK1
Fragment: 156-267
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.2
PUBMED ID: 36115835