Interaction Details


Details of experiments between P35222 and P57105
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CTNNB1
Fragment: 772-781
Site: PBM
Sequence: NQLAWFDTDL
Construct: biotin-ttds-NQLAWFDTDL

Protein B

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.17
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.09
PUBMED ID: 36115835