Interaction Details


Details of experiments between P33402 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GUCY1A2
Fragment: 723-732
Site: PBM
Sequence: GTMFLRETSL
Construct: biotin-ado-ado-GTMFLRETSL

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.46
Normalized holdup BI: 0.54
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.86
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: GUCY1A2
Fragment: 723-732
Site: PBM
Sequence: GTMFLRETSL
Construct: biotin-ado-ado-GTMFLRETSL

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.28
Normalized holdup BI: 0.34
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.5
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: GUCY1A2
Fragment: 723-732
Site: PBM
Sequence: GTMFLRETSL
Construct: biotin-ado-ado-GTMFLRETSL

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.