Interaction Details


Details of experiments between P31947 and Q9UQB8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 331-340
Site: 14-3-3_pSer336
Modification: (SEP)336
Sequence: SKLSDSYSNT
Construct: SKLSD(pSer)YSNT (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 113
P value: 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 331-340
Site: 14-3-3_pSer336
Modification: (SEP)336
Sequence: SKLSDSYSNT
Construct: SKLSD(pSer)YSNT (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 0.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 331-340
Site: 14-3-3_pSer336
Modification: (SEP)336
Sequence: SKLSDSYSNT
Construct: SKLSD(pSer)YSNT (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 1.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 361-370
Site: 14-3-3_pSer366
Modification: (SEP)366
Sequence: LPRSSSMAAG
Construct: LPRSS(pSer)MAAG (crude synthetic peptide)
Average holdup BI: 0.46
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 113
P value: 3.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.88
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 361-370
Site: 14-3-3_pSer366
Modification: (SEP)366
Sequence: LPRSSSMAAG
Construct: LPRSS(pSer)MAAG (crude synthetic peptide)
Average holdup BI: 0.38
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 5.46
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.63
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 361-370
Site: 14-3-3_pSer366
Modification: (SEP)366
Sequence: LPRSSSMAAG
Construct: LPRSS(pSer)MAAG (crude synthetic peptide)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.36
Immobilized partner concentration (10-6M): 111
P value: 0.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 514-523
Site: 14-3-3_pSer519
Modification: (SEP)519
Sequence: SGTLVSTV--
Construct: SGSGTLV(pSer)TV (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 113
P value: 0.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 514-523
Site: 14-3-3_pSer519
Modification: (SEP)519
Sequence: SGTLVSTV--
Construct: SGSGTLV(pSer)TV (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 142
P value: 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 514-523
Site: 14-3-3_pSer519
Modification: (SEP)519
Sequence: SGTLVSTV--
Construct: SGSGTLV(pSer)TV (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.24
Immobilized partner concentration (10-6M): 111
P value: 0.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 335-344
Site: 14-3-3_pThr340
Modification: (TPO)340
Sequence: DSYSNTLPVR
Construct: DSYSN(pThr)LPVR (crude synthetic peptide)
Average holdup BI: 0.68
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 7.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.16
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 335-344
Site: 14-3-3_pThr340
Modification: (TPO)340
Sequence: DSYSNTLPVR
Construct: DSYSN(pThr)LPVR (crude synthetic peptide)
Average holdup BI: 0.59
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 113
P value: 8.67
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.11
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 335-344
Site: 14-3-3_pThr340
Modification: (TPO)340
Sequence: DSYSNTLPVR
Construct: DSYSN(pThr)LPVR (crude synthetic peptide)
Average holdup BI: 0.56
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 111
P value: 6.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.05
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 355-364
Site: 14-3-3_pThr360
Modification: (TPO)360
Sequence: TTENKTLPRS
Construct: TTENK(pThr)LPRS (crude synthetic peptide)
Average holdup BI: -0.09
Holdup BI standard deviation: 0.52
Immobilized partner concentration (10-6M): 113
P value: 0.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 355-364
Site: 14-3-3_pThr360
Modification: (TPO)360
Sequence: TTENKTLPRS
Construct: TTENK(pThr)LPRS (crude synthetic peptide)
Average holdup BI: 0.09
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 142
P value: 0.86
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 15

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 355-364
Site: 14-3-3_pThr360
Modification: (TPO)360
Sequence: TTENKTLPRS
Construct: TTENK(pThr)LPRS (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 111
P value: 0.71
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 16

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 515-524
Site: 14-3-3_pThr520
Modification: (TPO)520
Sequence: GTLVSTV---
Construct: SGSGTLVS(pThr)V (crude synthetic peptide)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 113
P value: 0.99
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 515-524
Site: 14-3-3_pThr520
Modification: (TPO)520
Sequence: GTLVSTV---
Construct: SGSGTLVS(pThr)V (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 142
P value: 0.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 18

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: BAIAP2
Fragment: 515-524
Site: 14-3-3_pThr520
Modification: (TPO)520
Sequence: GTLVSTV---
Construct: SGSGTLVS(pThr)V (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 111
P value: 0.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))