Interaction Details


Details of experiments between P31947 and Q9BZF9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 1348-1357
Site: 14-3-3_pSer1353
Modification: (SEP)1353
Sequence: PTKRQSQLID
Construct: PTKRQ(pSer)QLID (crude synthetic peptide)
Average holdup BI: -0.17
Holdup BI standard deviation: 0.29
Immobilized partner concentration (10-6M): 113
P value: 0.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 1348-1357
Site: 14-3-3_pSer1353
Modification: (SEP)1353
Sequence: PTKRQSQLID
Construct: PTKRQ(pSer)QLID (crude synthetic peptide)
Average holdup BI: -0.14
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 142
P value: 1.28
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 1348-1357
Site: 14-3-3_pSer1353
Modification: (SEP)1353
Sequence: PTKRQSQLID
Construct: PTKRQ(pSer)QLID (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.22
Immobilized partner concentration (10-6M): 111
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 621-630
Site: 14-3-3_pSer626
Modification: (SEP)626
Sequence: AKLALSIPAE
Construct: AKLAL(pSer)IPAE (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 113
P value: 0.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 621-630
Site: 14-3-3_pSer626
Modification: (SEP)626
Sequence: AKLALSIPAE
Construct: AKLAL(pSer)IPAE (crude synthetic peptide)
Average holdup BI: -0.11
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 142
P value: 0.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 621-630
Site: 14-3-3_pSer626
Modification: (SEP)626
Sequence: AKLALSIPAE
Construct: AKLAL(pSer)IPAE (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 111
P value: 0.47
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 1043-1052
Site: 14-3-3_pThr1048
Modification: (TPO)1048
Sequence: DLRDKTVLIE
Construct: DLRDK(pThr)VLIE (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 113
P value: 0.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 1043-1052
Site: 14-3-3_pThr1048
Modification: (TPO)1048
Sequence: DLRDKTVLIE
Construct: DLRDK(pThr)VLIE (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 142
P value: 0.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: UACA
Fragment: 1043-1052
Site: 14-3-3_pThr1048
Modification: (TPO)1048
Sequence: DLRDKTVLIE
Construct: DLRDK(pThr)VLIE (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 111
P value: 0.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))