Interaction Details


Details of experiments between P31947 and Q96GV9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MACIR
Fragment: 127-136
Site: 14-3-3_pSer132
Modification: (SEP)132
Sequence: RRRMPSSGDK
Construct: RRRMP(pSer)SGDK (crude synthetic peptide)
Average holdup BI: -0.07
Holdup BI standard deviation: 0.26
Immobilized partner concentration (10-6M): 113
P value: 0.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MACIR
Fragment: 127-136
Site: 14-3-3_pSer132
Modification: (SEP)132
Sequence: RRRMPSSGDK
Construct: RRRMP(pSer)SGDK (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.65
Immobilized partner concentration (10-6M): 111
P value: 0.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MACIR
Fragment: 162-171
Site: 14-3-3_pSer167
Modification: (SEP)167
Sequence: RAHSKSLDYL
Construct: RAHSK(pSer)LDYL (crude synthetic peptide)
Average holdup BI: 0.83
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 113
P value: 5.52
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.62
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MACIR
Fragment: 162-171
Site: 14-3-3_pSer167
Modification: (SEP)167
Sequence: RAHSKSLDYL
Construct: RAHSK(pSer)LDYL (crude synthetic peptide)
Average holdup BI: 0.58
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 111
P value: 3.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.1
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))