Interaction Details


Details of experiments between P31947 and Q92625
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 24-33
Site: 14-3-3_pSer29
Modification: (SEP)29
Sequence: SGKRLSSGFG
Construct: SGKRL(pSer)SGFG (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 0.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 24-33
Site: 14-3-3_pSer29
Modification: (SEP)29
Sequence: SGKRLSSGFG
Construct: SGKRL(pSer)SGFG (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 1.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 24-33
Site: 14-3-3_pSer29
Modification: (SEP)29
Sequence: SGKRLSSGFG
Construct: SGKRL(pSer)SGFG (crude synthetic peptide)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 111
P value: 1.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 331-340
Site: 14-3-3_pSer336
Modification: (SEP)336
Sequence: SISQKSQGDV
Construct: SISQK(pSer)QGDV (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 113
P value: 0.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 331-340
Site: 14-3-3_pSer336
Modification: (SEP)336
Sequence: SISQKSQGDV
Construct: SISQK(pSer)QGDV (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 1.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 331-340
Site: 14-3-3_pSer336
Modification: (SEP)336
Sequence: SISQKSQGDV
Construct: SISQK(pSer)QGDV (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 111
P value: 0.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 621-630
Site: 14-3-3_pSer626
Modification: (SEP)626
Sequence: RSLSKSDSDL
Construct: RSLSK(pSer)DSDL (crude synthetic peptide)
Average holdup BI: 0.68
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 113
P value: 7.8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.28
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 621-630
Site: 14-3-3_pSer626
Modification: (SEP)626
Sequence: RSLSKSDSDL
Construct: RSLSK(pSer)DSDL (crude synthetic peptide)
Average holdup BI: 0.69
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 142
P value: 3.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.2
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 621-630
Site: 14-3-3_pSer626
Modification: (SEP)626
Sequence: RSLSKSDSDL
Construct: RSLSK(pSer)DSDL (crude synthetic peptide)
Average holdup BI: 0.73
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 111
P value: 6.85
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.39
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 658-667
Site: 14-3-3_pSer663
Modification: (SEP)663
Sequence: LEKSPSFASE
Construct: LEKSP(pSer)FASE (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 113
P value: 1.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 658-667
Site: 14-3-3_pSer663
Modification: (SEP)663
Sequence: LEKSPSFASE
Construct: LEKSP(pSer)FASE (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 142
P value: 1.65
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 658-667
Site: 14-3-3_pSer663
Modification: (SEP)663
Sequence: LEKSPSFASE
Construct: LEKSP(pSer)FASE (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 111
P value: 0.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 672-681
Site: 14-3-3_pSer677
Modification: (SEP)677
Sequence: EKIMSSIGEG
Construct: EKIMS(pSer)IGEG (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 113
P value: 1.61
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 672-681
Site: 14-3-3_pSer677
Modification: (SEP)677
Sequence: EKIMSSIGEG
Construct: EKIMS(pSer)IGEG (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 142
P value: 0.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 15

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 672-681
Site: 14-3-3_pSer677
Modification: (SEP)677
Sequence: EKIMSSIGEG
Construct: EKIMS(pSer)IGEG (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 111
P value: 0.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 16

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 840-849
Site: 14-3-3_pSer845
Modification: (SEP)845
Sequence: -PPRFSQLRC
Construct: PPRF(pSer)QLRCQ (crude synthetic peptide)
Average holdup BI: -0.12
Holdup BI standard deviation: 0.78
Immobilized partner concentration (10-6M): 113
P value: 0.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 840-849
Site: 14-3-3_pSer845
Modification: (SEP)845
Sequence: -PPRFSQLRC
Construct: PPRF(pSer)QLRCQ (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.8
Immobilized partner concentration (10-6M): 142
P value: 0.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 18

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 455-464
Site: 14-3-3_pThr460
Modification: (TPO)460
Sequence: YELLLTAETK
Construct: YELLL(pThr)AETK (crude synthetic peptide)
Average holdup BI: -0.15
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 113
P value: 0.9
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 19

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: ANKS1A
Fragment: 455-464
Site: 14-3-3_pThr460
Modification: (TPO)460
Sequence: YELLLTAETK
Construct: YELLL(pThr)AETK (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 111
P value: 0.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))