Interaction Details


Details of experiments between P31947 and Q86SQ0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 195-204
Site: 14-3-3_pSer200
Modification: (SEP)200
Sequence: RSGAASMPSS
Construct: RSGAA(pSer)MPSS (crude synthetic peptide)
Average holdup BI: 0.73
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 113
P value: 4.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.38
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 195-204
Site: 14-3-3_pSer200
Modification: (SEP)200
Sequence: RSGAASMPSS
Construct: RSGAA(pSer)MPSS (crude synthetic peptide)
Average holdup BI: 0.76
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 142
P value: 9.7
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.34
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 195-204
Site: 14-3-3_pSer200
Modification: (SEP)200
Sequence: RSGAASMPSS
Construct: RSGAA(pSer)MPSS (crude synthetic peptide)
Average holdup BI: 0.63
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 111
P value: 3.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.18
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 207-216
Site: 14-3-3_pSer212
Modification: (SEP)212
Sequence: QARKMSIQDS
Construct: QARKM(pSer)IQDS (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.24
Immobilized partner concentration (10-6M): 113
P value: 0.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 207-216
Site: 14-3-3_pSer212
Modification: (SEP)212
Sequence: QARKMSIQDS
Construct: QARKM(pSer)IQDS (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 142
P value: 0.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 207-216
Site: 14-3-3_pSer212
Modification: (SEP)212
Sequence: QARKMSIQDS
Construct: QARKM(pSer)IQDS (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.23
Immobilized partner concentration (10-6M): 111
P value: 0.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 237-246
Site: 14-3-3_pSer242
Modification: (SEP)242
Sequence: RTRKYSSSSL
Construct: RTRKY(pSer)SSSL (crude synthetic peptide)
Average holdup BI: 0.09
Holdup BI standard deviation: 0.61
Immobilized partner concentration (10-6M): 113
P value: 0.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 237-246
Site: 14-3-3_pSer242
Modification: (SEP)242
Sequence: RTRKYSSSSL
Construct: RTRKY(pSer)SSSL (crude synthetic peptide)
Average holdup BI: -0.08
Holdup BI standard deviation: 0.56
Immobilized partner concentration (10-6M): 142
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 272-281
Site: 14-3-3_pSer277
Modification: (SEP)277
Sequence: LPPRNSLGNS
Construct: LPPRN(pSer)LGNS (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 1.54
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 272-281
Site: 14-3-3_pSer277
Modification: (SEP)277
Sequence: LPPRNSLGNS
Construct: LPPRN(pSer)LGNS (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 142
P value: 0.98
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 272-281
Site: 14-3-3_pSer277
Modification: (SEP)277
Sequence: LPPRNSLGNS
Construct: LPPRN(pSer)LGNS (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 111
P value: 2.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 379-388
Site: 14-3-3_pSer384
Modification: (SEP)384
Sequence: TRRNFSCGSV
Construct: TRRNF(pSer)CGSV (crude synthetic peptide)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 111
P value: 0.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 410-419
Site: 14-3-3_pSer415
Modification: (SEP)415
Sequence: RERKSSISSI
Construct: RERKS(pSer)ISSI (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 142
P value: 0.29
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 463-472
Site: 14-3-3_pSer468
Modification: (SEP)468
Sequence: PDSRLSTGTT
Construct: PDSRL(pSer)TGTT (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 113
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 463-472
Site: 14-3-3_pSer468
Modification: (SEP)468
Sequence: PDSRLSTGTT
Construct: PDSRL(pSer)TGTT (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 0.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 16

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 463-472
Site: 14-3-3_pSer468
Modification: (SEP)468
Sequence: PDSRLSTGTT
Construct: PDSRL(pSer)TGTT (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 111
P value: 1.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 484-493
Site: 14-3-3_pSer489
Modification: (SEP)489
Sequence: EKLQLSDEES
Construct: EKLQL(pSer)DEES (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 113
P value: 0.28
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 484-493
Site: 14-3-3_pSer489
Modification: (SEP)489
Sequence: EKLQLSDEES
Construct: EKLQL(pSer)DEES (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 142
P value: 0.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 19

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 484-493
Site: 14-3-3_pSer489
Modification: (SEP)489
Sequence: EKLQLSDEES
Construct: EKLQL(pSer)DEES (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 111
P value: 0.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 20

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 1123-1132
Site: 14-3-3_pThr1128
Modification: (TPO)1128
Sequence: RSHVETAGHN
Construct: RSHVE(pThr)AGHN (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.38
Immobilized partner concentration (10-6M): 142
P value: 0.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 21

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 893-902
Site: 14-3-3_pThr898
Modification: (TPO)898
Sequence: LYLSDTLPRK
Construct: LYLSD(pThr)LPRK (crude synthetic peptide)
Average holdup BI: -0.12
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 113
P value: 1.84
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 22

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 893-902
Site: 14-3-3_pThr898
Modification: (TPO)898
Sequence: LYLSDTLPRK
Construct: LYLSD(pThr)LPRK (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 142
P value: 0.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 23

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PHLDB2
Fragment: 893-902
Site: 14-3-3_pThr898
Modification: (TPO)898
Sequence: LYLSDTLPRK
Construct: LYLSD(pThr)LPRK (crude synthetic peptide)
Average holdup BI: 0.12
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 111
P value: 1.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))