Interaction Details


Details of experiments between P31947 and Q7Z6B7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SRGAP1
Fragment: 492-501
Site: 14-3-3_pSer497
Modification: (SEP)497
Sequence: KSRPRSQYNT
Construct: KSRPR(pSer)QYNT (crude synthetic peptide)
Average holdup BI: -0.49
Holdup BI standard deviation: 0.99
Immobilized partner concentration (10-6M): 113
P value: 0.72
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SRGAP1
Fragment: 935-944
Site: 14-3-3_pSer940
Modification: (SEP)940
Sequence: IRRSTSSGQY
Construct: IRRST(pSer)SGQY (crude synthetic peptide)
Average holdup BI: 0.21
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 111
P value: 1.88
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))