Interaction Details


Details of experiments between P31947 and Q7L8J4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 228-237
Site: 14-3-3_pSer233
Modification: (SEP)233
Sequence: LKAQFSQILE
Construct: LKAQF(pSer)QILE (crude synthetic peptide)
Average holdup BI: -0.14
Holdup BI standard deviation: 0.25
Immobilized partner concentration (10-6M): 113
P value: 0.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 228-237
Site: 14-3-3_pSer233
Modification: (SEP)233
Sequence: LKAQFSQILE
Construct: LKAQF(pSer)QILE (crude synthetic peptide)
Average holdup BI: -0.47
Holdup BI standard deviation: 0.42
Immobilized partner concentration (10-6M): 142
P value: 1.84
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 228-237
Site: 14-3-3_pSer233
Modification: (SEP)233
Sequence: LKAQFSQILE
Construct: LKAQF(pSer)QILE (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 111
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 338-347
Site: 14-3-3_pSer343
Modification: (SEP)343
Sequence: LRTVASDLQK
Construct: LRTVA(pSer)DLQK (crude synthetic peptide)
Average holdup BI: 0.09
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 2.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 338-347
Site: 14-3-3_pSer343
Modification: (SEP)343
Sequence: LRTVASDLQK
Construct: LRTVA(pSer)DLQK (crude synthetic peptide)
Average holdup BI: 0.09
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 142
P value: 1.65
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 338-347
Site: 14-3-3_pSer343
Modification: (SEP)343
Sequence: LRTVASDLQK
Construct: LRTVA(pSer)DLQK (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 111
P value: 0.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 345-354
Site: 14-3-3_pSer350
Modification: (SEP)350
Sequence: LQKCDSVEHL
Construct: LQKCD(pSer)VEHL (crude synthetic peptide)
Average holdup BI: -0.2
Holdup BI standard deviation: 0.88
Immobilized partner concentration (10-6M): 113
P value: 0.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 357-366
Site: 14-3-3_pSer362
Modification: (SEP)362
Sequence: LSDHVSLDGQ
Construct: LSDHV(pSer)LDGQ (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 0.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 357-366
Site: 14-3-3_pSer362
Modification: (SEP)362
Sequence: LSDHVSLDGQ
Construct: LSDHV(pSer)LDGQ (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 0.87
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SH3BP5L
Fragment: 357-366
Site: 14-3-3_pSer362
Modification: (SEP)362
Sequence: LSDHVSLDGQ
Construct: LSDHV(pSer)LDGQ (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 111
P value: 0.36
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))