Interaction Details


Details of experiments between P31947 and Q6P597
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 168-177
Site: 14-3-3_pSer173
Modification: (SEP)173
Sequence: PPRRDSLASL
Construct: PPRRD(pSer)LASL (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 0.78
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 168-177
Site: 14-3-3_pSer173
Modification: (SEP)173
Sequence: PPRRDSLASL
Construct: PPRRD(pSer)LASL (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 142
P value: 0.36
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 168-177
Site: 14-3-3_pSer173
Modification: (SEP)173
Sequence: PPRRDSLASL
Construct: PPRRD(pSer)LASL (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 111
P value: 0.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: MKRAMSLNTL
Construct: MKRAM(pSer)LNTL (crude synthetic peptide)
Average holdup BI: 0.74
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 113
P value: 5.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.41
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: MKRAMSLNTL
Construct: MKRAM(pSer)LNTL (crude synthetic peptide)
Average holdup BI: 0.78
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 7.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.41
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: MKRAMSLNTL
Construct: MKRAM(pSer)LNTL (crude synthetic peptide)
Average holdup BI: 0.71
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 111
P value: 3.52
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.35
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 492-501
Site: 14-3-3_pSer497
Modification: (SEP)497
Sequence: RTLSASTQDL
Construct: RTLSA(pSer)TQDL (crude synthetic peptide)
Average holdup BI: 0.23
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 4.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.33
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 492-501
Site: 14-3-3_pSer497
Modification: (SEP)497
Sequence: RTLSASTQDL
Construct: RTLSA(pSer)TQDL (crude synthetic peptide)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 3.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC3
Fragment: 492-501
Site: 14-3-3_pSer497
Modification: (SEP)497
Sequence: RTLSASTQDL
Construct: RTLSA(pSer)TQDL (crude synthetic peptide)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 3.74
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))