Interaction Details


Details of experiments between P31947 and Q16875
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 17-26
Site: 14-3-3_pSer22
Modification: (SEP)22
Sequence: VDHRPSLPRS
Construct: VDHRP(pSer)LPRS (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 113
P value: 1.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 17-26
Site: 14-3-3_pSer22
Modification: (SEP)22
Sequence: VDHRPSLPRS
Construct: VDHRP(pSer)LPRS (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 142
P value: 0.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 17-26
Site: 14-3-3_pSer22
Modification: (SEP)22
Sequence: VDHRPSLPRS
Construct: VDHRP(pSer)LPRS (crude synthetic peptide)
Average holdup BI: -0.12
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 111
P value: 1.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 456-465
Site: 14-3-3_pSer461
Modification: (SEP)461
Sequence: LMRRNSVTPL
Construct: LMRRN(pSer)VTPL (crude synthetic peptide)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.18
Immobilized partner concentration (10-6M): 113
P value: 0.7
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 456-465
Site: 14-3-3_pSer461
Modification: (SEP)461
Sequence: LMRRNSVTPL
Construct: LMRRN(pSer)VTPL (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 142
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 456-465
Site: 14-3-3_pSer461
Modification: (SEP)461
Sequence: LMRRNSVTPL
Construct: LMRRN(pSer)VTPL (crude synthetic peptide)
Average holdup BI: -0.29
Holdup BI standard deviation: 0.5
Immobilized partner concentration (10-6M): 111
P value: 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 473-482
Site: 14-3-3_pSer478
Modification: (SEP)478
Sequence: KPRINSFEEH
Construct: KPRIN(pSer)FEEH (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 113
P value: 1.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 473-482
Site: 14-3-3_pSer478
Modification: (SEP)478
Sequence: KPRINSFEEH
Construct: KPRIN(pSer)FEEH (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 1.28
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: PFKFB3
Fragment: 473-482
Site: 14-3-3_pSer478
Modification: (SEP)478
Sequence: KPRINSFEEH
Construct: KPRIN(pSer)FEEH (crude synthetic peptide)
Average holdup BI: -0.11
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 111
P value: 0.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))