Interaction Details


Details of experiments between P31947 and Q15276
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 157-166
Site: 14-3-3_pSer162
Modification: (SEP)162
Sequence: LRRRLSEGQE
Construct: LRRRL(pSer)EGQE (crude synthetic peptide)
Average holdup BI: -0.17
Holdup BI standard deviation: 0.23
Immobilized partner concentration (10-6M): 113
P value: 0.8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 157-166
Site: 14-3-3_pSer162
Modification: (SEP)162
Sequence: LRRRLSEGQE
Construct: LRRRL(pSer)EGQE (crude synthetic peptide)
Average holdup BI: -0.39
Holdup BI standard deviation: 0.69
Immobilized partner concentration (10-6M): 142
P value: 0.66
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 157-166
Site: 14-3-3_pSer162
Modification: (SEP)162
Sequence: LRRRLSEGQE
Construct: LRRRL(pSer)EGQE (crude synthetic peptide)
Average holdup BI: -0.38
Holdup BI standard deviation: 0.9
Immobilized partner concentration (10-6M): 111
P value: 0.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 372-381
Site: 14-3-3_pSer377
Modification: (SEP)377
Sequence: RGSVHSLDAG
Construct: RGSVH(pSer)LDAG (crude synthetic peptide)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 113
P value: 0.89
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 372-381
Site: 14-3-3_pSer377
Modification: (SEP)377
Sequence: RGSVHSLDAG
Construct: RGSVH(pSer)LDAG (crude synthetic peptide)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 142
P value: 0.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 372-381
Site: 14-3-3_pSer377
Modification: (SEP)377
Sequence: RGSVHSLDAG
Construct: RGSVH(pSer)LDAG (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.19
Immobilized partner concentration (10-6M): 111
P value: 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 402-411
Site: 14-3-3_pSer407
Modification: (SEP)407
Sequence: LRRAQSTDSL
Construct: LRRAQ(pSer)TDSL (crude synthetic peptide)
Average holdup BI: 0.52
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 142
P value: 9.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.88
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 402-411
Site: 14-3-3_pSer407
Modification: (SEP)407
Sequence: LRRAQSTDSL
Construct: LRRAQ(pSer)TDSL (crude synthetic peptide)
Average holdup BI: 0.44
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 5.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.84
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 402-411
Site: 14-3-3_pSer407
Modification: (SEP)407
Sequence: LRRAQSTDSL
Construct: LRRAQ(pSer)TDSL (crude synthetic peptide)
Average holdup BI: 0.42
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 111
P value: 7.39
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.82
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 411-420
Site: 14-3-3_pSer416
Modification: (SEP)416
Sequence: LGTSGSLQSK
Construct: LGTSG(pSer)LQSK (crude synthetic peptide)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 113
P value: 1.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 411-420
Site: 14-3-3_pSer416
Modification: (SEP)416
Sequence: LGTSGSLQSK
Construct: LGTSG(pSer)LQSK (crude synthetic peptide)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 142
P value: 5.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 411-420
Site: 14-3-3_pSer416
Modification: (SEP)416
Sequence: LGTSGSLQSK
Construct: LGTSG(pSer)LQSK (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 1.39
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 425-434
Site: 14-3-3_pSer430
Modification: (SEP)430
Sequence: NYKAKSAGNL
Construct: NYKAK(pSer)AGNL (crude synthetic peptide)
Average holdup BI: 0.81
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 5.84
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.48
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 425-434
Site: 14-3-3_pSer430
Modification: (SEP)430
Sequence: NYKAKSAGNL
Construct: NYKAK(pSer)AGNL (crude synthetic peptide)
Average holdup BI: 0.74
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 4.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 425-434
Site: 14-3-3_pSer430
Modification: (SEP)430
Sequence: NYKAKSAGNL
Construct: NYKAK(pSer)AGNL (crude synthetic peptide)
Average holdup BI: 0.7
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 4.29
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.33
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 16

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 408-417
Site: 14-3-3_pThr413
Modification: (TPO)413
Sequence: TDSLGTSGSL
Construct: TDSLG(pThr)SGSL (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 113
P value: 0.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 408-417
Site: 14-3-3_pThr413
Modification: (TPO)413
Sequence: TDSLGTSGSL
Construct: TDSLG(pThr)SGSL (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 142
P value: 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 18

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RABEP1
Fragment: 408-417
Site: 14-3-3_pThr413
Modification: (TPO)413
Sequence: TDSLGTSGSL
Construct: TDSLG(pThr)SGSL (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 111
P value: 0.85
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))