Interaction Details


Details of experiments between P31947 and Q12840
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5A
Fragment: 150-159
Site: 14-3-3_pSer155
Modification: (SEP)155
Sequence: TKTNLSVHED
Construct: TKTNL(pSer)VHED (crude synthetic peptide)
Average holdup BI: -0.14
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 113
P value: 0.84
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5A
Fragment: 150-159
Site: 14-3-3_pSer155
Modification: (SEP)155
Sequence: TKTNLSVHED
Construct: TKTNL(pSer)VHED (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 142
P value: 0.86
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5A
Fragment: 150-159
Site: 14-3-3_pSer155
Modification: (SEP)155
Sequence: TKTNLSVHED
Construct: TKTNL(pSer)VHED (crude synthetic peptide)
Average holdup BI: -0.2
Holdup BI standard deviation: 0.23
Immobilized partner concentration (10-6M): 111
P value: 0.86
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5A
Fragment: 802-811
Site: 14-3-3_pThr807
Modification: (TPO)807
Sequence: VQDVTTRVKK
Construct: VQDVT(pThr)RVKK (crude synthetic peptide)
Average holdup BI: -0.11
Holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 113
P value: 0.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5A
Fragment: 802-811
Site: 14-3-3_pThr807
Modification: (TPO)807
Sequence: VQDVTTRVKK
Construct: VQDVT(pThr)RVKK (crude synthetic peptide)
Average holdup BI: -0.11
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 142
P value: 0.72
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5A
Fragment: 802-811
Site: 14-3-3_pThr807
Modification: (TPO)807
Sequence: VQDVTTRVKK
Construct: VQDVT(pThr)RVKK (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 111
P value: 0.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))