Interaction Details


Details of experiments between P31947 and Q08AE8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SPIRE1
Fragment: 459-468
Site: 14-3-3_pSer464
Modification: (SEP)464
Sequence: LAELDSSESE
Construct: LAELD(pSer)SESE (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.73
Immobilized partner concentration (10-6M): 113
P value: 0.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SPIRE1
Fragment: 459-468
Site: 14-3-3_pSer464
Modification: (SEP)464
Sequence: LAELDSSESE
Construct: LAELD(pSer)SESE (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.27
Immobilized partner concentration (10-6M): 111
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SPIRE1
Fragment: 453-462
Site: 14-3-3_pThr458
Modification: (TPO)458
Sequence: LLRAPTLAEL
Construct: LLRAP(pThr)LAEL (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.28
Immobilized partner concentration (10-6M): 113
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: SPIRE1
Fragment: 453-462
Site: 14-3-3_pThr458
Modification: (TPO)458
Sequence: LLRAPTLAEL
Construct: LLRAP(pThr)LAEL (crude synthetic peptide)
Average holdup BI: -0.09
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 111
P value: 1.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))