Interaction Details


Details of experiments between P31947 and Q07866
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 440-449
Site: 14-3-3_pSer445
Modification: (SEP)445
Sequence: QKDGTSFGEY
Construct: QKDGT(pSer)FGEY (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 0.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 440-449
Site: 14-3-3_pSer445
Modification: (SEP)445
Sequence: QKDGTSFGEY
Construct: QKDGT(pSer)FGEY (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 142
P value: 0.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 440-449
Site: 14-3-3_pSer445
Modification: (SEP)445
Sequence: QKDGTSFGEY
Construct: QKDGT(pSer)FGEY (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 111
P value: 0.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 516-525
Site: 14-3-3_pSer521
Modification: (SEP)521
Sequence: MEKRRSRESL
Construct: MEKRR(pSer)RESL (crude synthetic peptide)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.86
Immobilized partner concentration (10-6M): 142
P value: 0.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 516-525
Site: 14-3-3_pSer521
Modification: (SEP)521
Sequence: MEKRRSRESL
Construct: MEKRR(pSer)RESL (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 1.54
Immobilized partner concentration (10-6M): 111
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 519-528
Site: 14-3-3_pSer524
Modification: (SEP)524
Sequence: RRSRESLNVD
Construct: RRSRE(pSer)LNVD (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 113
P value: 0.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 519-528
Site: 14-3-3_pSer524
Modification: (SEP)524
Sequence: RRSRESLNVD
Construct: RRSRE(pSer)LNVD (crude synthetic peptide)
Average holdup BI: -0.14
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 3.25
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 519-528
Site: 14-3-3_pSer524
Modification: (SEP)524
Sequence: RRSRESLNVD
Construct: RRSRE(pSer)LNVD (crude synthetic peptide)
Average holdup BI: -0.25
Holdup BI standard deviation: 0.36
Immobilized partner concentration (10-6M): 111
P value: 0.85
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 529-538
Site: 14-3-3_pSer534
Modification: (SEP)534
Sequence: VVKYESGPDG
Construct: VVKYE(pSer)GPDG (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 113
P value: 0.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 529-538
Site: 14-3-3_pSer534
Modification: (SEP)534
Sequence: VVKYESGPDG
Construct: VVKYE(pSer)GPDG (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 142
P value: 0.24
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 529-538
Site: 14-3-3_pSer534
Modification: (SEP)534
Sequence: VVKYESGPDG
Construct: VVKYE(pSer)GPDG (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 111
P value: 0.8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 556-565
Site: 14-3-3_pSer561
Modification: (SEP)561
Sequence: LKRSGSFSKL
Construct: LKRSG(pSer)FSKL (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 113
P value: 0.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 556-565
Site: 14-3-3_pSer561
Modification: (SEP)561
Sequence: LKRSGSFSKL
Construct: LKRSG(pSer)FSKL (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 142
P value: 0.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 556-565
Site: 14-3-3_pSer561
Modification: (SEP)561
Sequence: LKRSGSFSKL
Construct: LKRSG(pSer)FSKL (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 111
P value: 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 563-572
Site: 14-3-3_pSer568
Modification: (SEP)568
Sequence: SKLRASIRRS
Construct: SKLRA(pSer)IRRS (crude synthetic peptide)
Average holdup BI: -0.33
Holdup BI standard deviation: 0.32
Immobilized partner concentration (10-6M): 142
P value: 1.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 16

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 563-572
Site: 14-3-3_pSer568
Modification: (SEP)568
Sequence: SKLRASIRRS
Construct: SKLRA(pSer)IRRS (crude synthetic peptide)
Average holdup BI: -0.46
Holdup BI standard deviation: 0.25
Immobilized partner concentration (10-6M): 111
P value: 2.24
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 595-604
Site: 14-3-3_pSer600
Modification: (SEP)600
Sequence: MKRASSLNVL
Construct: MKRAS(pSer)LNVL (crude synthetic peptide)
Average holdup BI: 0.68
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 6.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.27
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 595-604
Site: 14-3-3_pSer600
Modification: (SEP)600
Sequence: MKRASSLNVL
Construct: MKRAS(pSer)LNVL (crude synthetic peptide)
Average holdup BI: 0.72
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 7.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.26
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 19

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 595-604
Site: 14-3-3_pSer600
Modification: (SEP)600
Sequence: MKRASSLNVL
Construct: MKRAS(pSer)LNVL (crude synthetic peptide)
Average holdup BI: 0.66
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 5.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.24
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 20

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 626-635
Site: 14-3-3_pSer631
Modification: (SEP)631
Sequence: RALSASHTDL
Construct: RALSA(pSer)HTDL (crude synthetic peptide)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 113
P value: 3.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 21

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 626-635
Site: 14-3-3_pSer631
Modification: (SEP)631
Sequence: RALSASHTDL
Construct: RALSA(pSer)HTDL (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 3.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 22

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KLC1
Fragment: 626-635
Site: 14-3-3_pSer631
Modification: (SEP)631
Sequence: RALSASHTDL
Construct: RALSA(pSer)HTDL (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 111
P value: 3.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))