Interaction Details


Details of experiments between P31947 and Q07002
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 127-136
Site: 14-3-3_pSer132
Modification: (SEP)132
Sequence: MSRRASLSDI
Construct: MSRRA(pSer)LSDI (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 113
P value: 0.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 127-136
Site: 14-3-3_pSer132
Modification: (SEP)132
Sequence: MSRRASLSDI
Construct: MSRRA(pSer)LSDI (crude synthetic peptide)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 142
P value: 3.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 127-136
Site: 14-3-3_pSer132
Modification: (SEP)132
Sequence: MSRRASLSDI
Construct: MSRRA(pSer)LSDI (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.37
Immobilized partner concentration (10-6M): 111
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 11-20
Site: 14-3-3_pSer16
Modification: (SEP)16
Sequence: RRFSLSVPRT
Construct: RRFSL(pSer)VPRT (crude synthetic peptide)
Average holdup BI: 0.68
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 142
P value: 3.99
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.18
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 11-20
Site: 14-3-3_pSer16
Modification: (SEP)16
Sequence: RRFSLSVPRT
Construct: RRFSL(pSer)VPRT (crude synthetic peptide)
Average holdup BI: 0.78
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 4.83
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.51
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 84-93
Site: 14-3-3_pSer89
Modification: (SEP)89
Sequence: NQRRFSMEDV
Construct: NQRRF(pSer)MEDV (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.18
Immobilized partner concentration (10-6M): 113
P value: 0
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 84-93
Site: 14-3-3_pSer89
Modification: (SEP)89
Sequence: NQRRFSMEDV
Construct: NQRRF(pSer)MEDV (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 142
P value: 0.48
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 84-93
Site: 14-3-3_pSer89
Modification: (SEP)89
Sequence: NQRRFSMEDV
Construct: NQRRF(pSer)MEDV (crude synthetic peptide)
Average holdup BI: -0.15
Holdup BI standard deviation: 0.87
Immobilized partner concentration (10-6M): 111
P value: 0.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 93-102
Site: 14-3-3_pSer98
Modification: (SEP)98
Sequence: VSKRLSLPMD
Construct: VSKRL(pSer)LPMD (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 142
P value: 1.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 93-102
Site: 14-3-3_pSer98
Modification: (SEP)98
Sequence: VSKRLSLPMD
Construct: VSKRL(pSer)LPMD (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.25
Immobilized partner concentration (10-6M): 111
P value: 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 152-161
Site: 14-3-3_pThr157
Modification: (TPO)157
Sequence: EGTYATVFKG
Construct: EGTYA(pThr)VFKG (crude synthetic peptide)
Average holdup BI: 0.31
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 5.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.49
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 152-161
Site: 14-3-3_pThr157
Modification: (TPO)157
Sequence: EGTYATVFKG
Construct: EGTYA(pThr)VFKG (crude synthetic peptide)
Average holdup BI: 0.25
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 5.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.46
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: CDK18
Fragment: 152-161
Site: 14-3-3_pThr157
Modification: (TPO)157
Sequence: EGTYATVFKG
Construct: EGTYA(pThr)VFKG (crude synthetic peptide)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 3.6
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))