Interaction Details


Details of experiments between P31947 and P33176
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 149-158
Site: 14-3-3_pSer154
Modification: (SEP)154
Sequence: SKTNLSVHED
Construct: SKTNL(pSer)VHED (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 113
P value: 1.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 149-158
Site: 14-3-3_pSer154
Modification: (SEP)154
Sequence: SKTNLSVHED
Construct: SKTNL(pSer)VHED (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 0.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 149-158
Site: 14-3-3_pSer154
Modification: (SEP)154
Sequence: SKTNLSVHED
Construct: SKTNL(pSer)VHED (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 111
P value: 0.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 933-942
Site: 14-3-3_pSer938
Modification: (SEP)938
Sequence: SPTHPSAIRG
Construct: SPTHP(pSer)AIRG (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 113
P value: 0.37
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 933-942
Site: 14-3-3_pSer938
Modification: (SEP)938
Sequence: SPTHPSAIRG
Construct: SPTHP(pSer)AIRG (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 142
P value: 0.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 933-942
Site: 14-3-3_pSer938
Modification: (SEP)938
Sequence: SPTHPSAIRG
Construct: SPTHP(pSer)AIRG (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 111
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 945-954
Site: 14-3-3_pSer950
Modification: (SEP)950
Sequence: AFVQNSQPVA
Construct: AFVQN(pSer)QPVA (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 113
P value: 3.89
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 945-954
Site: 14-3-3_pSer950
Modification: (SEP)950
Sequence: AFVQNSQPVA
Construct: AFVQN(pSer)QPVA (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 142
P value: 1.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 945-954
Site: 14-3-3_pSer950
Modification: (SEP)950
Sequence: AFVQNSQPVA
Construct: AFVQN(pSer)QPVA (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 111
P value: 0.85
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 392-401
Site: 14-3-3_pThr397
Modification: (TPO)397
Sequence: VDKDITLTND
Construct: VDKDI(pThr)LTND (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 1.45
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 392-401
Site: 14-3-3_pThr397
Modification: (TPO)397
Sequence: VDKDITLTND
Construct: VDKDI(pThr)LTND (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 142
P value: 0.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 392-401
Site: 14-3-3_pThr397
Modification: (TPO)397
Sequence: VDKDITLTND
Construct: VDKDI(pThr)LTND (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 111
P value: 0.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 446-455
Site: 14-3-3_pThr451
Modification: (TPO)451
Sequence: VEKLKTQMLD
Construct: VEKLK(pThr)QMLD (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 113
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 446-455
Site: 14-3-3_pThr451
Modification: (TPO)451
Sequence: VEKLKTQMLD
Construct: VEKLK(pThr)QMLD (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 142
P value: 0.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 15

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: KIF5B
Fragment: 446-455
Site: 14-3-3_pThr451
Modification: (TPO)451
Sequence: VEKLKTQMLD
Construct: VEKLK(pThr)QMLD (crude synthetic peptide)
Average holdup BI: -0.16
Holdup BI standard deviation: 0.73
Immobilized partner concentration (10-6M): 111
P value: 0.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))