Interaction Details


Details of experiments between P31947 and P04049
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RAF1
Fragment: 254-264
Site: 14-3-3_pSer259
Modification: (SEP)259
Sequence: RQRSTSTPNV
Construct: RQRST(pSer)TPNVH (crude synthetic peptide)
Average holdup BI: 0.61
Holdup BI standard deviation: 0.45
Immobilized partner concentration (10-6M): 142
P value: 1.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.04
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RAF1
Fragment: 254-264
Site: 14-3-3_pSer259
Modification: (SEP)259
Sequence: RQRSTSTPNV
Construct: RQRST(pSer)TPNVH (crude synthetic peptide)
Average holdup BI: -0.18
Holdup BI standard deviation: 0.72
Immobilized partner concentration (10-6M): 113
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: RAF1
Fragment: 254-264
Site: 14-3-3_pSer259
Modification: (SEP)259
Sequence: RQRSTSTPNV
Construct: RQRST(pSer)TPNVH (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.54
Immobilized partner concentration (10-6M): 111
P value: 0.25
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))