Interaction Details


Details of experiments between P31947 and O94915
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 98-107
Site: 14-3-3_pSer103
Modification: (SEP)103
Sequence: RSSTKSKGDE
Construct: RSSTK(pSer)KGDE (crude synthetic peptide)
Average holdup BI: -0.3
Holdup BI standard deviation: 0.56
Immobilized partner concentration (10-6M): 113
P value: 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 98-107
Site: 14-3-3_pSer103
Modification: (SEP)103
Sequence: RSSTKSKGDE
Construct: RSSTK(pSer)KGDE (crude synthetic peptide)
Average holdup BI: -0.3
Holdup BI standard deviation: 0.57
Immobilized partner concentration (10-6M): 142
P value: 0.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1909-1918
Site: 14-3-3_pSer1914
Modification: (SEP)1914
Sequence: AANRKSTGQL
Construct: AANRK(pSer)TGQL (crude synthetic peptide)
Measured dissociation constant (10-6M): <100
Immobilized partner concentration (10-6M): 142
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 1
Measured pKd: 4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1909-1918
Site: 14-3-3_pSer1914
Modification: (SEP)1914
Sequence: AANRKSTGQL
Construct: AANRK(pSer)TGQL (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 113
P value: 0.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1930-1939
Site: 14-3-3_pSer1935
Modification: (SEP)1935
Sequence: YLGYNSNARS
Construct: YLGYN(pSer)NARS (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1930-1939
Site: 14-3-3_pSer1935
Modification: (SEP)1935
Sequence: YLGYNSNARS
Construct: YLGYN(pSer)NARS (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 142
P value: 1.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1930-1939
Site: 14-3-3_pSer1935
Modification: (SEP)1935
Sequence: YLGYNSNARS
Construct: YLGYN(pSer)NARS (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 0.38
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1936-1945
Site: 14-3-3_pSer1941
Modification: (SEP)1941
Sequence: NARSNSLRLS
Construct: NARSN(pSer)LRLS (crude synthetic peptide)
Average holdup BI: 0.35
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 113
P value: 6.66
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1936-1945
Site: 14-3-3_pSer1941
Modification: (SEP)1941
Sequence: NARSNSLRLS
Construct: NARSN(pSer)LRLS (crude synthetic peptide)
Average holdup BI: 0.39
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 6.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.65
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1936-1945
Site: 14-3-3_pSer1941
Modification: (SEP)1941
Sequence: NARSNSLRLS
Construct: NARSN(pSer)LRLS (crude synthetic peptide)
Average holdup BI: 0.31
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 4.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1940-1949
Site: 14-3-3_pSer1945
Modification: (SEP)1945
Sequence: NSLRLSLIGD
Construct: NSLRL(pSer)LIGD (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 113
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1940-1949
Site: 14-3-3_pSer1945
Modification: (SEP)1945
Sequence: NSLRLSLIGD
Construct: NSLRL(pSer)LIGD (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 111
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1967-1976
Site: 14-3-3_pSer1972
Modification: (SEP)1972
Sequence: INHSSSLART
Construct: INHSS(pSer)LART (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 0.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1967-1976
Site: 14-3-3_pSer1972
Modification: (SEP)1972
Sequence: INHSSSLART
Construct: INHSS(pSer)LART (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 142
P value: 0.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 15

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1967-1976
Site: 14-3-3_pSer1972
Modification: (SEP)1972
Sequence: INHSSSLART
Construct: INHSS(pSer)LART (crude synthetic peptide)
Average holdup BI: -0.08
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 111
P value: 0.77
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 16

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1973-1982
Site: 14-3-3_pSer1978
Modification: (SEP)1978
Sequence: LARTRSLSSL
Construct: LARTR(pSer)LSSL (crude synthetic peptide)
Average holdup BI: 0.75
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 8.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.32
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 17

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1973-1982
Site: 14-3-3_pSer1978
Modification: (SEP)1978
Sequence: LARTRSLSSL
Construct: LARTR(pSer)LSSL (crude synthetic peptide)
Average holdup BI: 0.69
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 5.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.3
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1973-1982
Site: 14-3-3_pSer1978
Modification: (SEP)1978
Sequence: LARTRSLSSL
Construct: LARTR(pSer)LSSL (crude synthetic peptide)
Average holdup BI: 0.7
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 111
P value: 6.16
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.32
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 19

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1954-1963
Site: 14-3-3_pThr1959
Modification: (TPO)1959
Sequence: RRRSNTLDIM
Construct: RRRSN(pThr)LDIM (crude synthetic peptide)
Average holdup BI: 0.6
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 113
P value: 1.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.11
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 20

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1954-1963
Site: 14-3-3_pThr1959
Modification: (TPO)1959
Sequence: RRRSNTLDIM
Construct: RRRSN(pThr)LDIM (crude synthetic peptide)
Average holdup BI: 0.57
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 142
P value: 3.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.97
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 21

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 1954-1963
Site: 14-3-3_pThr1959
Modification: (TPO)1959
Sequence: RRRSNTLDIM
Construct: RRRSN(pThr)LDIM (crude synthetic peptide)
Measured dissociation constant (10-6M): <100
Immobilized partner concentration (10-6M): 111
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 1
Measured pKd: 4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 22

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 477-486
Site: 14-3-3_pThr482
Modification: (TPO)482
Sequence: LPSGNTLRVK
Construct: LPSGN(pThr)LRVK (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 0.61
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 23

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 477-486
Site: 14-3-3_pThr482
Modification: (TPO)482
Sequence: LPSGNTLRVK
Construct: LPSGN(pThr)LRVK (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 142
P value: 0.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 24

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: FRYL
Fragment: 477-486
Site: 14-3-3_pThr482
Modification: (TPO)482
Sequence: LPSGNTLRVK
Construct: LPSGN(pThr)LRVK (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 0.73
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))