Interaction Details


Details of experiments between P31947 and O43379
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1129-1138
Site: 14-3-3_pSer1134
Modification: (SEP)1134
Sequence: MDRGGSQPRA
Construct: MDRGG(pSer)QPRA (crude synthetic peptide)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.35
Immobilized partner concentration (10-6M): 142
P value: 0.45
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1129-1138
Site: 14-3-3_pSer1134
Modification: (SEP)1134
Sequence: MDRGGSQPRA
Construct: MDRGG(pSer)QPRA (crude synthetic peptide)
Average holdup BI: -0.17
Holdup BI standard deviation: 1.24
Immobilized partner concentration (10-6M): 111
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1223-1232
Site: 14-3-3_pSer1228
Modification: (SEP)1228
Sequence: ISRSISLGDS
Construct: ISRSI(pSer)LGDS (crude synthetic peptide)
Average holdup BI: 0.64
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 142
P value: 7.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.09
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1223-1232
Site: 14-3-3_pSer1228
Modification: (SEP)1228
Sequence: ISRSISLGDS
Construct: ISRSI(pSer)LGDS (crude synthetic peptide)
Average holdup BI: 0.57
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 6.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.06
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1223-1232
Site: 14-3-3_pSer1228
Modification: (SEP)1228
Sequence: ISRSISLGDS
Construct: ISRSI(pSer)LGDS (crude synthetic peptide)
Average holdup BI: 0.54
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 111
P value: 7.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.02
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1244-1253
Site: 14-3-3_pSer1249
Modification: (SEP)1249
Sequence: LRRPSSVGEL
Construct: LRRPS(pSer)VGEL (crude synthetic peptide)
Average holdup BI: 0.29
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 142
P value: 3.74
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.46
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1244-1253
Site: 14-3-3_pSer1249
Modification: (SEP)1249
Sequence: LRRPSSVGEL
Construct: LRRPS(pSer)VGEL (crude synthetic peptide)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 113
P value: 2.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1244-1253
Site: 14-3-3_pSer1249
Modification: (SEP)1249
Sequence: LRRPSSVGEL
Construct: LRRPS(pSer)VGEL (crude synthetic peptide)
Average holdup BI: 0.3
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 111
P value: 5.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.58
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1337-1346
Site: 14-3-3_pSer1342
Modification: (SEP)1342
Sequence: SAFRPSLPAP
Construct: SAFRP(pSer)LPAP (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 113
P value: 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1337-1346
Site: 14-3-3_pSer1342
Modification: (SEP)1342
Sequence: SAFRPSLPAP
Construct: SAFRP(pSer)LPAP (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 142
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 1337-1346
Site: 14-3-3_pSer1342
Modification: (SEP)1342
Sequence: SAFRPSLPAP
Construct: SAFRP(pSer)LPAP (crude synthetic peptide)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 111
P value: 1.83
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 44-53
Site: 14-3-3_pSer49
Modification: (SEP)49
Sequence: RRTRLSTASE
Construct: RRTRL(pSer)TASE (crude synthetic peptide)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 142
P value: 1.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 892-901
Site: 14-3-3_pSer897
Modification: (SEP)897
Sequence: ESLENSILDS
Construct: ESLEN(pSer)ILDS (crude synthetic peptide)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 113
P value: 0.7
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: WDR62
Fragment: 892-901
Site: 14-3-3_pSer897
Modification: (SEP)897
Sequence: ESLENSILDS
Construct: ESLEN(pSer)ILDS (crude synthetic peptide)
Average holdup BI: -0.21
Holdup BI standard deviation: 0.27
Immobilized partner concentration (10-6M): 142
P value: 1.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))