Interaction Details


Details of experiments between P31947 and Q7KZI7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 371-380
Site: 14-3-3_pSer376
Modification: (SEP)376
Sequence: KQRRFSDQAG
Construct: KQRRF(pSer)DQAG (crude synthetic peptide)
Average holdup BI: -0.35
Holdup BI standard deviation: 0.18
Immobilized partner concentration (10-6M): 111
P value: 1.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 35-44
Site: 14-3-3_pSer40
Modification: (SEP)40
Sequence: IRGRNSATSA
Construct: IRGRN(pSer)ATSA (crude synthetic peptide)
Average holdup BI: 0.92
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 111
P value: 2.39
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.03
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 395-404
Site: 14-3-3_pSer400
Modification: (SEP)400
Sequence: VQRSVSANPK
Construct: VQRSV(pSer)ANPK (crude synthetic peptide)
Average holdup BI: 0.82
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 3.96
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.5
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 395-404
Site: 14-3-3_pSer400
Modification: (SEP)400
Sequence: VQRSVSANPK
Construct: VQRSV(pSer)ANPK (crude synthetic peptide)
Average holdup BI: 0.59
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 113
P value: 2.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.11
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 404-413
Site: 14-3-3_pSer409
Modification: (SEP)409
Sequence: KQRRFSDQAA
Construct: KQRRF(pSer)DQAA (crude synthetic peptide)
Average holdup BI: 0.31
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 142
P value: 4.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.5
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 488-497
Site: 14-3-3_pSer493
Modification: (SEP)493
Sequence: LLERASLGQA
Construct: LLERA(pSer)LGQA (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 488-497
Site: 14-3-3_pSer493
Modification: (SEP)493
Sequence: LLERASLGQA
Construct: LLERA(pSer)LGQA (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 142
P value: 0.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 488-497
Site: 14-3-3_pSer493
Modification: (SEP)493
Sequence: LLERASLGQA
Construct: LLERA(pSer)LGQA (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 111
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 566-575
Site: 14-3-3_pSer571
Modification: (SEP)571
Sequence: PVASPSAHNI
Construct: PVASP(pSer)AHNI (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 1.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 566-575
Site: 14-3-3_pSer571
Modification: (SEP)571
Sequence: PVASPSAHNI
Construct: PVASP(pSer)AHNI (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 142
P value: 0.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 566-575
Site: 14-3-3_pSer571
Modification: (SEP)571
Sequence: PVASPSAHNI
Construct: PVASP(pSer)AHNI (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 111
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 626-635
Site: 14-3-3_pSer631
Modification: (SEP)631
Sequence: GRRGASGSIF
Construct: GRRGA(pSer)GSIF (crude synthetic peptide)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 113
P value: 2.54
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 626-635
Site: 14-3-3_pSer631
Modification: (SEP)631
Sequence: GRRGASGSIF
Construct: GRRGA(pSer)GSIF (crude synthetic peptide)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 142
P value: 1.98
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 14

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 626-635
Site: 14-3-3_pSer631
Modification: (SEP)631
Sequence: GRRGASGSIF
Construct: GRRGA(pSer)GSIF (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 111
P value: 1.85
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 319-328
Site: 14-3-3_pThr324
Modification: (TPO)324
Sequence: NEVMATYLLL
Construct: NEVMA(pThr)YLLL (crude synthetic peptide)
Average holdup BI: -0.3
Holdup BI standard deviation: 0.65
Immobilized partner concentration (10-6M): 111
P value: 0.43
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 16

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 461-470
Site: 14-3-3_pThr466
Modification: (TPO)466
Sequence: LERKKTTPTP
Construct: LERKK(pThr)TPTP (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 113
P value: 1.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 461-470
Site: 14-3-3_pThr466
Modification: (TPO)466
Sequence: LERKKTTPTP
Construct: LERKK(pThr)TPTP (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 142
P value: 0.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 18

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 461-470
Site: 14-3-3_pThr466
Modification: (TPO)466
Sequence: LERKKTTPTP
Construct: LERKK(pThr)TPTP (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 111
P value: 0.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 19

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 53-62
Site: 14-3-3_pThr58
Modification: (TPO)58
Sequence: YRLLKTIGKG
Construct: YRLLK(pThr)IGKG (crude synthetic peptide)
Average holdup BI: 0.27
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 142
P value: 2.78
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.41
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 20

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 53-62
Site: 14-3-3_pThr58
Modification: (TPO)58
Sequence: YRLLKTIGKG
Construct: YRLLK(pThr)IGKG (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.18
Immobilized partner concentration (10-6M): 113
P value: 0.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 21

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 53-62
Site: 14-3-3_pThr58
Modification: (TPO)58
Sequence: YRLLKTIGKG
Construct: YRLLK(pThr)IGKG (crude synthetic peptide)
Average holdup BI: 0.46
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 111
P value: 3.46
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.89
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 22

Protein A

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma

Protein B

Protein: MARK2
Fragment: 591-600
Site: 14-3-3_pThr596
Modification: (TPO)596
Sequence: VSSRSTFHAG
Construct: VSSRS(pThr)FHAG (crude synthetic peptide)
Average holdup BI: 0.52
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 113
P value: 1.78
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.99
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))