Interaction Details


Details of experiments between P31946 and Q8WUI4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 150-159
Site: 14-3-3_pSer155
Modification: (SEP)155
Sequence: LRKTVSEPNL
Construct: LRKTV(pSer)EPNL (crude synthetic peptide)
Average holdup BI: 0.97
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 8.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.37
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 150-159
Site: 14-3-3_pSer155
Modification: (SEP)155
Sequence: LRKTVSEPNL
Construct: LRKTV(pSer)EPNL (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 102
P value: 8.36
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.12
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 176-185
Site: 14-3-3_pSer181
Modification: (SEP)181
Sequence: LLRKESAPPS
Construct: LLRKE(pSer)APPS (crude synthetic peptide)
Average holdup BI: 0.27
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 102
P value: 6.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.56
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 176-185
Site: 14-3-3_pSer181
Modification: (SEP)181
Sequence: LLRKESAPPS
Construct: LLRKE(pSer)APPS (crude synthetic peptide)
Average holdup BI: 0.26
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 4.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 353-362
Site: 14-3-3_pSer358
Modification: (SEP)358
Sequence: LSRTRSEPLP
Construct: LSRTR(pSer)EPLP (crude synthetic peptide)
Average holdup BI: 0.86
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 5.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.67
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 353-362
Site: 14-3-3_pSer358
Modification: (SEP)358
Sequence: LSRTRSEPLP
Construct: LSRTR(pSer)EPLP (crude synthetic peptide)
Average holdup BI: 0.58
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 102
P value: 6.25
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.12
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 400-409
Site: 14-3-3_pSer405
Modification: (SEP)405
Sequence: LRQIPSAEDL
Construct: LRQIP(pSer)AEDL (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 134
P value: 0.54
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 400-409
Site: 14-3-3_pSer405
Modification: (SEP)405
Sequence: LRQIPSAEDL
Construct: LRQIP(pSer)AEDL (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 102
P value: 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 459-468
Site: 14-3-3_pSer464
Modification: (SEP)464
Sequence: RLPRGSTGDT
Construct: RLPRG(pSer)TGDT (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.18
Immobilized partner concentration (10-6M): 134
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 459-468
Site: 14-3-3_pSer464
Modification: (SEP)464
Sequence: RLPRGSTGDT
Construct: RLPRG(pSer)TGDT (crude synthetic peptide)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.28
Immobilized partner concentration (10-6M): 102
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 481-490
Site: 14-3-3_pSer486
Modification: (SEP)486
Sequence: LSRAQSSPAA
Construct: LSRAQ(pSer)SPAA (crude synthetic peptide)
Average holdup BI: 0.97
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 102
P value: 10.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.47
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 481-490
Site: 14-3-3_pSer486
Modification: (SEP)486
Sequence: LSRAQSSPAA
Construct: LSRAQ(pSer)SPAA (crude synthetic peptide)
Average holdup BI: 0.97
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 134
P value: 7.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.35
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 502-511
Site: 14-3-3_pSer507
Modification: (SEP)507
Sequence: QARVLSSSET
Construct: QARVL(pSer)SSET (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 134
P value: 0.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: HDAC7
Fragment: 502-511
Site: 14-3-3_pSer507
Modification: (SEP)507
Sequence: QARVLSSSET
Construct: QARVL(pSer)SSET (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 102
P value: 0.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))