Interaction Details


Details of experiments between P31946 and Q5VV41
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: ARHGEF16
Fragment: 102-111
Site: 14-3-3_pSer107
Modification: (SEP)107
Sequence: PARHQSFGAA
Construct: PARHQ(pSer)FGAA (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 134
P value: 0.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: ARHGEF16
Fragment: 102-111
Site: 14-3-3_pSer107
Modification: (SEP)107
Sequence: PARHQSFGAA
Construct: PARHQ(pSer)FGAA (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.49
Immobilized partner concentration (10-6M): 102
P value: 0.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: ARHGEF16
Fragment: 487-496
Site: 14-3-3_pSer492
Modification: (SEP)492
Sequence: FSKVKSLPLI
Construct: FSKVK(pSer)LPLI (crude synthetic peptide)
Average holdup BI: 0.95
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 3.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.19
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: ARHGEF16
Fragment: 487-496
Site: 14-3-3_pSer492
Modification: (SEP)492
Sequence: FSKVKSLPLI
Construct: FSKVK(pSer)LPLI (crude synthetic peptide)
Average holdup BI: 0.92
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 102
P value: 5.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.04
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: ARHGEF16
Fragment: 1-10
Site: 14-3-3_pSer6
Modification: (SEP)6
Sequence: MAQRHSDSSL
Construct: MAQRH(pSer)DSSL (crude synthetic peptide)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.88
Immobilized partner concentration (10-6M): 102
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))