Interaction Details


Details of experiments between P31946 and Q13009
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 1402-1411
Site: 14-3-3_pSer1407
Modification: (SEP)1407
Sequence: LLKTESLPSS
Construct: LLKTE(pSer)LPSS (crude synthetic peptide)
Average holdup BI: 0.73
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 102
P value: 7.67
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.42
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 1402-1411
Site: 14-3-3_pSer1407
Modification: (SEP)1407
Sequence: LLKTESLPSS
Construct: LLKTE(pSer)LPSS (crude synthetic peptide)
Average holdup BI: 0.72
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 8.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.28
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 1432-1441
Site: 14-3-3_pSer1437
Modification: (SEP)1437
Sequence: MSRAVSAPSK
Construct: MSRAV(pSer)APSK (crude synthetic peptide)
Average holdup BI: 0.95
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 102
P value: 9.24
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.29
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 1432-1441
Site: 14-3-3_pSer1437
Modification: (SEP)1437
Sequence: MSRAVSAPSK
Construct: MSRAV(pSer)APSK (crude synthetic peptide)
Average holdup BI: 0.96
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 5.48
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.27
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 167-176
Site: 14-3-3_pSer172
Modification: (SEP)172
Sequence: KKRSKSADIW
Construct: KKRSK(pSer)ADIW (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.69
Immobilized partner concentration (10-6M): 134
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 167-176
Site: 14-3-3_pSer172
Modification: (SEP)172
Sequence: KKRSKSADIW
Construct: KKRSK(pSer)ADIW (crude synthetic peptide)
Average holdup BI: -0.19
Holdup BI standard deviation: 1.07
Immobilized partner concentration (10-6M): 102
P value: 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 329-338
Site: 14-3-3_pSer334
Modification: (SEP)334
Sequence: SEFADSGIEG
Construct: SEFAD(pSer)GIEG (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.52
Immobilized partner concentration (10-6M): 134
P value: 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 329-338
Site: 14-3-3_pSer334
Modification: (SEP)334
Sequence: SEFADSGIEG
Construct: SEFAD(pSer)GIEG (crude synthetic peptide)
Average holdup BI: -0.15
Holdup BI standard deviation: 0.59
Immobilized partner concentration (10-6M): 102
P value: 0.24
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 55-64
Site: 14-3-3_pSer60
Modification: (SEP)60
Sequence: STRSSSTPSI
Construct: STRSS(pSer)TPSI (crude synthetic peptide)
Average holdup BI: 0.76
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 102
P value: 13.54
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.49
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 55-64
Site: 14-3-3_pSer60
Modification: (SEP)60
Sequence: STRSSSTPSI
Construct: STRSS(pSer)TPSI (crude synthetic peptide)
Average holdup BI: 0.75
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 10.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.36
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 314-323
Site: 14-3-3_pThr319
Modification: (TPO)319
Sequence: GRRAKTTQDV
Construct: GRRAK(pThr)TQDV (crude synthetic peptide)
Average holdup BI: 0.8
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 102
P value: 5.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta

Protein B

Protein: TIAM1
Fragment: 314-323
Site: 14-3-3_pThr319
Modification: (TPO)319
Sequence: GRRAKTTQDV
Construct: GRRAK(pThr)TQDV (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.96
Immobilized partner concentration (10-6M): 134
P value: 0.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))