Interaction Details


Details of experiments between P30291 and Q8N5U6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: WEE1
Fragment: 228-247
Construct: Biotin-Ahx-RQTPQVNINPFTPDSLLLHS-CONH2

Protein B

Protein: RNF10
Fragment: 1-811
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.64
Immobilized partner concentration (10-6M): 10
P value: 1.93
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
Measured pKd: 5.25
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.12