Interaction Details


Details of experiments between P30260 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CDC27
Fragment: 1-824
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.69
Immobilized partner concentration (10-6M): 10
P value: 2.87
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.34
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 2

Protein A

Protein: CDC27
Fragment: 1-824
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-his6-MBP-TEVsite-SNX27
Average holdup BI: 0.67
Immobilized partner concentration (10-6M): 10
P value: 4.68
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.31
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 3

Protein A

Protein: CDC27
Fragment: 1-824
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.35
Immobilized partner concentration (10-6M): 10
P value: 6.73
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 4.73
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))