Interaction Details


Details of experiments between P30260 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.81
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5.47
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.81
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5.47
PUBMED ID: N

Experiment series 3

Protein A

Protein: CDC27
Fragment: 1-824
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.74
Immobilized partner concentration (10-6M): 10
P value: 4.31
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.46
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 4

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.74
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 5.29
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: CDC27
Fragment: 1-824
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 10
P value: 9.41
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.15
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 6

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.08
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.21
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 4.2
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.09
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0.07
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.5
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 5.32
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.47
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.12
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.46
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.58
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 4
Measured pKd: 4.91
PUBMED ID: N

Experiment series 14

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.72
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 5.22
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.69
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.15
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.58
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 4
Measured pKd: 4.92
PUBMED ID: N

Experiment series 17

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.54
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 5.41
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.52
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.12
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.59
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 4
Measured pKd: 4.96
PUBMED ID: N

Experiment series 20

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.77
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5.35
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.77
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5.35
PUBMED ID: N

Experiment series 22

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.72
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 5.22
PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.67
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.13
PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.69
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 5.16
PUBMED ID: 38995680

Experiment series 25

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.67
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.13
PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.63
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 4
Measured pKd: 5.02
PUBMED ID: N

Experiment series 27

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.61
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 9
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 5.37
PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.53
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.02
PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.19
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 4
Measured pKd: 4.14
PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.05
PUBMED ID: 38995680