Interaction Details


Details of experiments between P29074 and P23634
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PTPN4
Fragment: 513-603
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B4
Fragment: 1232-1241
Site: PBM
Sequence: SSLQSLETSV
Construct: biotin-ttds-SSLQSLETSV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.3
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 7
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.88
PUBMED ID: 36115835