Interaction Details


Details of experiments between P28223 and Q14005
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HTR2A
Fragment: 462-471
Site: PBM
Sequence: DGVNEKVSCV
Construct: biotin-ado-ado-DGVNEKVSCV

Protein B

Protein: IL16
Fragment: 210-306
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.46
Normalized holdup BI: 0.46
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.72
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: HTR2A
Fragment: 462-471
Site: PBM
Sequence: DGVNEKVSCV
Construct: biotin-ado-ado-DGVNEKVSCV

Protein B

Protein: IL16
Fragment: 1203-1332
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.56
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: HTR2A
Fragment: 462-471
Site: PBM
Sequence: DGVNEKVSCV
Construct: biotin-ado-ado-DGVNEKVSCV

Protein B

Protein: IL16
Fragment: 1106-1201
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.