Interaction Details


Details of experiments between P27816 and Q14160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAP4
Fragment: 1143-1152
Site: PBM
Sequence: LDSQIQETSI
Construct: biotin-ttds-LDSQIQETSI

Protein B

Protein: SCRIB
Fragment: 1-1630
Construct: endogenous protein (Jurkat)
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.97
PUBMED ID: 36542723

Experiment series 2

Protein A

Protein: MAP4
Fragment: 1143-1152
Site: PBM
Sequence: LDSQIQETSI
Construct: biotin-ttds-LDSQIQETSI

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.8
Normalized holdup BI: 0.84
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.56
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAP4
Fragment: 1143-1152
Site: PBM
Sequence: LDSQIQETSI
Construct: biotin-ttds-LDSQIQETSI

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.62
Normalized holdup BI: 0.69
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.17
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAP4
Fragment: 1143-1152
Site: PBM
Sequence: LDSQIQETSI
Construct: biotin-ttds-LDSQIQETSI

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.26
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.32
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.45
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAP4
Fragment: 1-1152
Construct: endogenous protein (Jurkat)

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 10
P value: 0.93
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 6

Protein A

Protein: MAP4
Fragment: 1-1152
Construct: endogenous protein (Jurkat)

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 10
P value: 0.15
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))