Interaction Details


Details of experiments between P27348 and Q8WUI4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau

Protein B

Protein: HDAC7
Fragment: 150-159
Site: 14-3-3_pSer155
Modification: (SEP)155
Sequence: LRKTVSEPNL
Construct: LRKTV(pSer)EPNL (crude synthetic peptide)
Average holdup BI: 0.92
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 130
P value: 8.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.95
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau

Protein B

Protein: HDAC7
Fragment: 176-185
Site: 14-3-3_pSer181
Modification: (SEP)181
Sequence: LLRKESAPPS
Construct: LLRKE(pSer)APPS (crude synthetic peptide)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 130
P value: 5.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.28
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau

Protein B

Protein: HDAC7
Fragment: 353-362
Site: 14-3-3_pSer358
Modification: (SEP)358
Sequence: LSRTRSEPLP
Construct: LSRTR(pSer)EPLP (crude synthetic peptide)
Average holdup BI: 0.67
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 130
P value: 4.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.19
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau

Protein B

Protein: HDAC7
Fragment: 400-409
Site: 14-3-3_pSer405
Modification: (SEP)405
Sequence: LRQIPSAEDL
Construct: LRQIP(pSer)AEDL (crude synthetic peptide)
Average holdup BI: -0.09
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 130
P value: 1.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau

Protein B

Protein: HDAC7
Fragment: 459-468
Site: 14-3-3_pSer464
Modification: (SEP)464
Sequence: RLPRGSTGDT
Construct: RLPRG(pSer)TGDT (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 130
P value: 0.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau

Protein B

Protein: HDAC7
Fragment: 481-490
Site: 14-3-3_pSer486
Modification: (SEP)486
Sequence: LSRAQSSPAA
Construct: LSRAQ(pSer)SPAA (crude synthetic peptide)
Average holdup BI: 0.96
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 130
P value: 10.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.26
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau

Protein B

Protein: HDAC7
Fragment: 502-511
Site: 14-3-3_pSer507
Modification: (SEP)507
Sequence: QARVLSSSET
Construct: QARVL(pSer)SSET (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))