Interaction Details


Details of experiments between P27348 and Q13094
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau

Protein B

Protein: LCP2
Fragment: 371-382
Site: 14-3-3_pSer376
Modification: (SEP)376
Sequence: FPQSASLPPY
Construct: FPQSA(pSer)LPPYFS (crude synthetic peptide)
Average holdup BI: 0.98
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 130
P value: 1.49
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.62
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))