Interaction Details


Details of experiments between P27228 and O14908
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV35-E6
Fragment: 140-149
Site: PBM
Sequence: SKPTRRETEV
Construct: biotin-ttds-SKPTRRETEV

Protein B

Protein: GIPC1
Fragment: 128-223
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 39.7
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.69
PUBMED ID: 36115835