Interaction Details


Details of experiments between P27228 and Q09666
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV35-E6
Fragment: 140-149
Site: PBM
Sequence: SKPTRRETEV
Construct: biotin-ttds-SKPTRRETEV

Protein B

Protein: AHNAK1
Fragment: 2-99
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Holdup BI standard deviation: 0.01
Normalized holdup BI: -0.03
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18.55
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV35-E6
Fragment: 140-149
Site: PBM
Modification: (TPO)147
Sequence: SKPTRRETEV
Construct: Biotin-ttds-SKPTRREpTEV

Protein B

Protein: AHNAK1
Fragment: 2-99
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835