Interaction Details


Details of experiments between P27037 and Q9H8Y8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL

Protein B

Protein: GORASP2
Fragment: 1-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Normalized holdup BI: 0.19
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.87
PUBMED ID: 36115835