Interaction Details


Details of experiments between P27037 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.43
Holdup BI standard deviation: 0.19
Normalized holdup BI: 0.43
Normalized holdup BI standard deviation: 0.19
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 4.38
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 47.52
Standard deviation of dissociation constant (10-6M): 4.66
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.32
PUBMED ID: 36115835