Interaction Details


Details of experiments between P26554 and Q8TDM6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV

Protein B

Protein: DLG5
Fragment: 714-800
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.63
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV

Protein B

Protein: DLG5
Fragment: 1336-1439
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV

Protein B

Protein: DLG5
Fragment: 1492-1595
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV

Protein B

Protein: DLG5
Fragment: 616-713
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV

Protein B

Protein: DLG5
Fragment: 616-713
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835