Interaction Details


Details of experiments between P26554 and O14640
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV

Protein B

Protein: DVL1
Fragment: 248-337
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.32
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 7.7
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.86
PUBMED ID: 36115835