Interaction Details


Details of experiments between P26045 and P27228
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PTPN3
Fragment: 505-597
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV35-E6
Fragment: 140-149
Site: PBM
Sequence: SKPTRRETEV
Construct: biotin-ttds-SKPTRRETEV
Average holdup BI: 0.58
Normalized holdup BI: 0.58
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.95
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: PTPN3
Fragment: 505-597
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV35-E6
Fragment: 140-149
Site: PBM
Sequence: SKPTRRETEV
Construct: biotin-ttds-SKPTRRETEV
Average holdup BI: 0.58
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.58
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18.55
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.93
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PTPN3
Fragment: 505-597
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV35-E6
Fragment: 140-149
Site: PBM
Sequence: SKPTRRETEV
Construct: biotin-ttds-SKPTRRETEV
Average holdup BI: 0.52
Normalized holdup BI: 0.52
Immobilized partner concentration (10-6M): 18.55
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.82
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: PTPN3
Fragment: 505-597
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV35-E6
Fragment: 140-149
Site: PBM
Sequence: SKPTRRETEV
Construct: biotin-ttds-SKPTRRETEV
Average holdup BI: 0.36
Immobilized partner concentration (10-6M): 39.7
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.17
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: PTPN3
Fragment: 505-597
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV35-E6
Fragment: 140-149
Site: PBM
Modification: (TPO)147
Sequence: SKPTRRETEV
Construct: Biotin-ttds-SKPTRREpTEV
Average holdup BI: 0.14
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 36115835