Interaction Details


Details of experiments between P26045 and O15169
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PTPN3
Fragment: 505-597
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: AXIN1
Fragment: 333-347
Site: Internal_PBM
Construct: Biotin-GSGSGS-TLSLTDSSVDGIPPYR-CONH2
Average holdup BI: 0.11
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 36115835