Interaction Details


Details of experiments between P25054 and Q92743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: APC
Fragment: 2834-2843
Site: PBM
Sequence: HSGSYLVTSV
Construct: Biotin-Ahx-HSGSYLVTSV

Protein B

Protein: HTRA1
Fragment: 365-480
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Normalized holdup BI: 0.22
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.23
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: APC
Fragment: 2834-2843
Site: PBM
Sequence: HSGSYLVTSV
Construct: Biotin-Ahx-HSGSYLVTSV

Protein B

Protein: HTRA1
Fragment: 365-480
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.21
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.2
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.