Interaction Details


Details of experiments between P23634 and Q9Y2H9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ATP2B4
Fragment: 1232-1241
Site: PBM
Sequence: SSLQSLETSV
Construct: biotin-ttds-SSLQSLETSV

Protein B

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.37
Normalized holdup BI: 0.73
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.26
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ATP2B4
Fragment: 1232-1241
Site: PBM
Sequence: SSLQSLETSV
Construct: biotin-ttds-SSLQSLETSV

Protein B

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.36
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: ATP2B4
Fragment: 1232-1241
Site: PBM
Sequence: SSLQSLETSV
Construct: biotin-ttds-SSLQSLETSV

Protein B

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Normalized holdup BI: 0.44
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.69
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6