Interaction Details


Details of experiments between P22460 and Q9NSN8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL

Protein B

Protein: SNTG1
Fragment: 54-143
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL

Protein B

Protein: SNTG1
Fragment: 54-143
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Normalized holdup BI: 0.4
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.6
PUBMED ID: 36115835