Interaction Details


Details of experiments between P20020 and Q9H5P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: Biotin-ado-ado-SPLHSLETSL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.56
Normalized holdup BI: 0.59
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.96
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: biotin-ttds-SPLHSLETSL

Protein B

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Normalized holdup BI: 0.41
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.63
PUBMED ID: 36115835