Interaction Details

Details of experiments between P20020 and Q7Z6J2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: Biotin-ado-ado-SPLHSLETSL

Protein B

Protein: GRASP
Fragment: 96-193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.24

Normalized holdup BI: 0.24

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.27

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: biotin-ttds-SPLHSLETSL