Interaction Details


Details of experiments between P17386 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV31-E6
Fragment: 140-149
Site: PBM
Sequence: SRRPRTETQV
Construct: biotin-ttds-SRRPRTETQV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.8
Holdup BI standard deviation: 0
Normalized holdup BI: 0.9
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.79
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV31-E6
Fragment: 140-149
Site: PBM
Sequence: SRRPRTETQV
Construct: biotin-ttds-SRRPRTETQV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.58
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.69
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.16
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV31-E6
Fragment: 140-149
Site: PBM
Sequence: SRRPRTETQV
Construct: biotin-ttds-SRRPRTETQV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.39
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.6
PUBMED ID: 36115835