Interaction Details


Details of experiments between P13569 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CFTR
Fragment: 1471-1480
Site: PBM
Sequence: TEEEVQDTRL
Construct: Biotin-ado-ado-TEEEVQDTRL

Protein B

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.59
Normalized holdup BI: 0.62
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.03
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: CFTR
Fragment: 1471-1480
Site: PBM
Sequence: TEEEVQDTRL
Construct: biotin-ttds-TEEEVQDTRL

Protein B

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.46
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.49
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.78
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: CFTR
Fragment: 1471-1480
Site: PBM
Sequence: TEEEVQDTRL
Construct: Biotin-ado-ado-TEEEVQDTRL

Protein B

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.25
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: CFTR
Fragment: 1471-1480
Site: PBM
Sequence: TEEEVQDTRL
Construct: biotin-ttds-TEEEVQDTRL

Protein B

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.08
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: CFTR
Fragment: 1471-1480
Site: PBM
Sequence: TEEEVQDTRL
Construct: Biotin-ado-ado-TEEEVQDTRL

Protein B

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.92
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: CFTR
Fragment: 1471-1480
Site: PBM
Sequence: TEEEVQDTRL
Construct: biotin-ttds-TEEEVQDTRL

Protein B

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Normalized holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.79
PUBMED ID: 36115835