Interaction Details

Details of experiments between P0DTC9 and Q8WXI2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: SARS-Cov2-N
Fragment: 410-419
Site: PBM
Sequence: SMSSADSTQA
Construct: Biotin-(PEG)3-QQSMSSADSTQA

Protein B

Protein: CNKSR2
Fragment: 208-305
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 20

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835