Interaction Details


Details of experiments between P0DTC9 and Q8TB45
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-Cov2-N
Fragment: 410-419
Site: PBM
Sequence: SMSSADSTQA
Construct: Biotin-(PEG)3-QQSMSSADSTQA

Protein B

Protein: DEPTOR
Fragment: 321-406
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835